cell culture

protein

This is a lyophilized protein: shipping at ambient temperature is recommended, for long term storage ( up to 2 years) at -20ºC or lower. Storage of reconstituted protein 1 month at +4ºC

Collagen Types in bones and muscles can be hydrolyzed and used in human grafts. ( Type II )

A gel is a solid jelly-like material that can have properties ranging from soft and weak to hard and tough. Gels are defined as a substantially dilute cross-linked system, which exhibits no flow when in the steady-state. By weight, gels are mostly liquid, yet they behave like solids due to a three-dimensional cross-linked network within the liquid. It is the crosslinking within the fluid that gives a gel its structure (hardness) and contributes to the adhesive stick (tack). In this way gels are a dispersion of molecules of a liquid within a solid in which the solid is the continuous phase and the liquid is the discontinuous phase. The word gel was coined by 19th-century Scottish chemist Thomas Graham by clipping from gelatin.Mouse or mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab. Mouse are mature after 40 days for females and 55 days for males. The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year. Transgenic, knock-out, congenic and inbread strains are known for C57BL/6, A/J, BALB/c, SCID while the CD-1 is outbred as strain.

The term “freeze-dry” is a lay description for the process of lyophilization protein powder. It is the conversion of water from a frozen state to a gaseous state without going through a liquid state. Aliquoting with 25 mM HEPES-KOH, pH 7.2 and 1 mM DTT and 10% weight on volume glycerol is suitable for storage of the reconstituted powder. The freeze-dry process removes moisture from the cells of specimens while the specimens remain frozen.

Mus musculus

This 1 has passed a sterilization (or sterilization) by filtration or inactivation that has eliminated (removed) or killed (deactivated) all forms of DNA, RNA and enzymes. Also life and other biological agents (such as viruses which some do not consider to be alive but are biological pathogens nonetheless), excluding prions which cannot be killed, including transmissible agents (such as fungi, bacteria, viruses, prions, spore forms, unicellular eukaryotic organisms such as Plasmodium, etc.) present in a specified reagent or on a surface, a volume of fluid, or in a compound such as biological culture medias filtered. Sterilization was achieved with one or more of the following heat, chemicals, irradiation, high pressure, and filtration. Sterilization is distinct from disinfection, sanitization, and pasteurization in that sterilization kills, deactivates, or eliminates all forms of life and other biological agents.