ELISA category
Affinity chromatography tool affinity gel
ELISA detection limits
N/A
ELISA's description
Kit for manufacturing of immuno affinity columns by immobilization of ligands with primary and secondary amines using activated cellulose beads; The BioTeZ-activated cellulose beads provide an easy way to immobilize ligands. The coupling reaction is simple to carry out. Many applications are possible, e.g. immobilization of proteins, peptides and nucleic acids. Precondition for coupling is that ligands contain primary and secondary amines. BioTeZ-activated cellulose beads enables customized immuno affinity gels and col-umns with the following properties: • reusable, • excellent flow behaviour without extra pressure, • little abrasion. The coupled product has a high chemical stability to commonly used aqueous solu-tions. Usable with non-ionic detergents, denaturing solvents, e.g. 6 M guanidine hydrochloride and 8 M urea. Stable in organic solvents, such as 50% dioxane and 50% dimethylformamide. To autoclave is not recommended.
Research area interests
all immuno assays
Package form
activated gel
Tested applications
Kit for preparation of individual immuno affinity columns for purification, enrichment or isolation of target molecules.
Preservative materials
If applicable mentioned in the product data sheet
Storage recommendations
12 month
Shipping and storage conditions
> 0 to +37°C
Assay principle
immobilization of ligands with primary and secondary amines using activated cellulose beads
Included in the standard package
Pre-activated cellulose beads, Coupling solution, Block solution, Wasch buffer 1 and 2, Storage buffer, empty columns
Not included in the standard package
Glass frit (G1 or G2), filter flask, pump/water-jet pump, pipettes or pipetting equipment, rotating mixer (recommended) , distilled or deionised water
Samples to be analyzed
immobilization of ligands with primary and secondary amines using activated cellulose beads
PubMed citations
See the datasheet
Technical datasheet
Contact us to receive the datasheet
Representative figures
No Available
Warnings
For Research Use only.
Test
A high affinity purification column was use to purify BioTeZ IAC Kit for column preparation, Pre-activated chromatography medium incl. empty columns chromatography tool by biotez by chromatographic size exclusion.A gel is a solid jelly-like material that can have properties ranging from soft and weak to hard and tough. Gels are defined as a substantially dilute cross-linked system, which exhibits no flow when in the steady-state. By weight, gels are mostly liquid, yet they behave like solids due to a three-dimensional cross-linked network within the liquid. It is the crosslinking within the fluid that gives a gel its structure (hardness) and contributes to the adhesive stick (tack). In this way gels are a dispersion of molecules of a liquid within a solid in which the solid is the continuous phase and the liquid is the discontinuous phase. The word gel was coined by 19th-century Scottish chemist Thomas Graham by clipping from gelatin.
Description
There are two major types of growth media those used for cell culture , which use specific cell types derived from plants or animals, and microbiological culture, which are used for growing microorganisms, such as bacteria or yeast. The most common growth media for microorganisms are nutrient broths and agar plates with antibiotics; specialized media are sometimes required for microorganism and cell culture growth.