PCR, polymerase chain reaction

TAQ or Pfu or Pfx or other enzymes are used for polycmerase chain reaction and have different specificity. The mores specific the lower the yield.Real-time PCR amplification mixes are available for quantification with specific selected primers and with optimized buffers.

Thermocyclers can be callibrated for identical ramping curves to obtain a more accurate PCR.

Real time PCR kits are used with DNA extraction kits supplied in 50 or 100 tests with polymerase chain reaction (PCR). It monitors the amplification of a targeted DNA molecule during the PCR quantitatively (Quantitative real-time PCR), semi-quantitatively. RNA is often converted to cDNA. Often used as diagnostic tool. Ct values will have to be set for your probes.