Assay Information
This Urinary Prostacyclin (UP) kit is a competitive immunoassay for the determination of Prostacyclin (PGI ) breakdown products in urine. Please read the complete kit insert before performing this assay. The kit measures the two major breakdown products of PGI in urine, 6ketoProstaglandin Flambdaalpha (6kPGFlambdaalpha ) and 2,3dinor6ketoProstaglandin Flambdaalpha (2,3d6kPGFlambdaalpha ). The kit uses a polyclonal antibody that binds these two molecules with almost equal affinities. This antibody binds in a competitive manner, both the 6kPGF and 2,3d6kPGF in the sample, or an alkaline phosphatase molecule which has 6kPGFlambdaalpha covalently attached to it. After a simultaneous incubation at room temperature the excess reagents are washed away and substrate is added. After a short incubation time the color generated is read on amicroplate reader at 405nm. The intensity of the bound yellow color is inversely proportional to the total concentration of 6kPGFlambdaalpha and 2,3d6kPGF in either standards or samples. Themeasured optical density of the 2,3d6kPGFlambdaalpha standards is used to calculate the concentration of both 6kPGFlambdaalpha and 2,3d6kPGFlambdaalpha in the sample.