Area of research
DNA & RNA
Store at 2-8 deg C
Optimal conditions to be determined by end-user
AntiSSA is an indirect solid phase enzyme immunometric assay (ELISA). It is designed for the quantitative measurement of IgG class autoantibodies directed against the extractable nuclear antigens SSA (Ro). The microplate is coated with SSA (Ro). The microplate can be divided into 12 modules of 8 wells each or can be used completely for 96 determinations. Each well can be separated from the module ("breakaway"). The wells of the microplate are stained with a specific color for each ENA antigen. The binding of present autoantibodies, as well as the formation of the sandwich complexes and enzymatic color reaction take place during three different reaction phases. To read the optical density a microplate reader with a 450 nm filter is required. Bichromatic measurement with a 600690 nm reference is recommended. The optical density for each calibrator may be graphically plotted against the concentration of IgG and unknowns extrapolated from the curve.
E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays,E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays
ELISA Enzyme-linked immunosorbent assays Code 90320007 SNOMED