Area of research
Differentiation & Development
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The AntiSpermatozoa Antibody ELISA (Enzyme Linked ImmunoSorbent Assay) IgClassifying Test from IBLAmerica is a solidphase sandwich enzymeimmunoassay for the quantitative determination of antispermatozoa antibodies in human serum. The ELISAplate is coated with a mix of spermatozoa proteins which are recognized by antispermatozoa antibodies. The samples and controls are pipetted into the wells and then incubated. During this incubation antispermatozoa antibodies bind to the spermatozoa proteins and are thus immobilized on the plate. An enzyme conjugate containing antiserum directed against different regions of human immunoglobulins of different classes (IgA, IgG, IgM) and POD binds to the antigenantibodycomplex during the incubation. After removal of the unbound conjugate by washing the horseradish peroxidase oxidizes the then added substrate TMB (3,3’,5,5’tetramethylbenzidine) yielding a color reaction which is stopped with 0.25 M sulphuric acid (H2SO4). The extinction is measured at a wavelength of 450 nm with a microplate reader. The use of a reference measurement with a wavelength 550 nm or higher is recommended.
E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays,E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays
ELISA Enzyme-linked immunosorbent assays Code 90320007 SNOMED