Area of research
Cytokines & Growth Factors
Store at 2-8 deg C
Optimal conditions to be determined by end user
An antiVEGFC polyclonal coating antibody is adsorbed onto microwells. VEGFC present in the sample or standard binds to antibodies adsorbed to the microwells; a biotin conjugated polyclonal VEGFC antibody is added and binds to VEGFC captured by the receptors. Following incubation unbound biotin conjugated VEGFC is removed during a wash step. StreptavidinHRP is added and binds to the biotin conjugated VEGFC. Following incubation unbound StreptavidinHRP is removed during a wash step, and substrate solution reactive with HRP is added to the wells. A coloured product is formed in proportion to the amount of VEGFC present in the sample. The reaction is terminated by addition of acid and absorbance is measured at 450 nm. A standard curve is prepared from seven VEGFC standard dilutions and VEGFC sample concentration determined.
E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays,E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays
ELISA Enzyme-linked immunosorbent assays Code 90320007 SNOMED
Rats are used to make rat monoclonal anti mouse antibodies. There are less rat- than mouse clones however. Rats genes from rodents of the genus Rattus norvegicus are often studied in vivo as a model of human genes in Sprague-Dawley or Wistar rats.