Area of research
Cytokines & Growth Factors
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Optimal conditions to be determined by end user
An antirat IL2 monoclonal coating antibody is adsorbed onto microwells. Rat IL2 present in the sample or standard binds to antibodies adsorbed to the microwells; a biotinconjugated monoclonal antirat IL2 antibody is added and binds to rat IFN gamma captured by the first antibody. Following incubation unbound biotin conjugated antirat IL2 is removed during a wash step. StreptavidinHRP is added and binds to the biotin conjugated antirat IL2. Following incubation unbound StreptavidinHRP is removed during a wash step, and substrate solution reactive with HRP is added to the wells. A coloured product is formed in proportion to the amount of rat IL2 present in the sample. The reaction is terminated by addition of acid and absorbance is measured at 450 nm. A standard curve is prepared from seven rat IL2 standard dilutions and rat IL2 sample concentration determined.
E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays,E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays
ELISA Enzyme-linked immunosorbent assays Code 90320007 SNOMED
Interleukin-2 (IL-2) is an interleukin, a type of cytokine signaling molecule in the immune system. It is a protein that regulates the activities of white blood cells (leukocytes, often lymphocytes) that are responsible for immunity. IL-2 is part of the body's natural response to microbial infection, and in discriminating between foreign ("non-self") and "self". IL-2 mediates its effects by binding to IL-2 receptors, which are expressed by lymphocytes. Rec. E. coli interleukin-2 for T cell culture or antibody production is supplied by GENTAUR. Free samples on request.
Rats are used to make rat monoclonal anti mouse antibodies. There are less rat- than mouse clones however. Rats genes from rodents of the genus Rattus norvegicus are often studied in vivo as a model of human genes in Sprague-Dawley or Wistar rats.