Optimal conditions to be determined by end user
The PSA ELISA test is a solid phase two-site immunoassay. An anti-PSA monoclonal antibody is coated on the surface of the microtiter wells and a mouse anti-PSA antibody labeled with horseradish peroxidase is used as the tracer. The PSA molecules present in the standard solution or sera are 'sandwiched' between the two antibodies. Following the formation of the coated antibody-antigen-antibody-enzyme complex, the unbound proteins and antibody-enzyme tracers are removed by washing. The horseradish peroxidase activity bound in the wells is then assayed by a colorimetric reaction. The intensity of the color formed is proportional to the concentration of PSA present in the sample.
E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays,E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays
ELISA Enzyme-linked immunosorbent assays Code 90320007 SNOMED