Area of research
Cytokines & Growth Factors
Storage
Store at 2-8 deg C
Shipping conditions
Blue Ice
Usage Recommendations
Optimal conditions to be determined by end user
Assay Information
An antipig IL18 coating antibody is adsorbed onto microwells. Pig IL18 present in the sample or standard binds to antibodies adsorbed to the microwells. A biotinconjugated antipig IL18 antibody is added and binds to pig IL18 captured by the first antibody. Following incubation unbound biotinconjugated antipig IL18 antibody is removed during a wash step. StreptavidinHRP is added and binds to the biotinconjugated antipig IL18 antibody. Following incubation unbound StreptavidinHRP is removed during a wash step, and substrate solution reactive with HRP is added to the wells. A coloured product is formed in proportion to the amount of pig IL18 present in the sample or standard. The reaction is terminated by addition of acid and absorbance is measured at 450 nm. A standard curve is prepared from 7 pig IL18 standard dilutions and pig IL18 sample concentration determined.
Properties
E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays,E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays
Test
ELISA Enzyme-linked immunosorbent assays Code 90320007 SNOMED
About
Pigs and the smaller guinea pigs are frequent used as models for humans.