All suppliers fitzgerald phosphoMEK1 ELISA kit

Information

Catalog number
55R-IB09643
Name
phosphoMEK1 ELISA kit
Supplier
Size
96 wells
Price
1217.00

Details

Category
Research kit
Area of research
Signal Transduction
Storage
Store at 2-8 deg C
Shipping conditions
Blue Ice
Tested for
ELISA
Usage Recommendations
Optimal conditions to be determined by end user
Assay Information
This phosphoMEK 1 (pMEK 1) Enzyme Immunometric Assay (EIA) kit is a complete for the determination of pMEK1 in cell lysates. Please read the complete kit insert before performingthis assay. The kit uses amonoclonal antibody toMEK1 immobilized on amicrotiter plate to bind the pMEK 1 in the standards or samples. A recombinant phosphorylated MEK 1 Standard is provided in the kit. After a short incubation the excess sample or standard is washed out and a rabbit polyclonal antibody to pMEK 1/2 is added. This antibody binds to the pMEK1 captured on the plate. After a short incubation the excess antibody is washed out and antirabbit IgG conjugated to Horseradish peroxidase is added,which binds to the polyclonal pMEK1/2 antibody. Excess conjugate is washed out and substrate is added. After a short incubation, the enzyme reaction is stopped and the color generated is read at 450 nm. The measured optical density is directly proportional to the concentration of pMEK1 in either standards or samples.
Properties
E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays,E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays
Test
ELISA Enzyme-linked immunosorbent assays Code 90320007 SNOMED