All suppliers fitzgerald Phospholipid Screen IgG/IgM ELISA kit

Information

Catalog number
55R-ORG529
Name
Phospholipid Screen IgG/IgM ELISA kit
Supplier
Size
96 wells
Price
427.00

Details

Category
Research kit
Area of research
Immunology
Storage
Store at 2-8 deg C
Shipping conditions
Blue Ice
Tested for
ELISA
Usage Recommendations
Optimal conditions to be determined by end-user
Assay Information
A mixture of highly purified Cardiolipin, Phosphatidyl Serine, Phosphatidyl Inositol, Phosphatidic Acid and human beta2Glycoprotein I is bound to microwells. Antibodies against these antigens, if present in diluted serum or plasma, bind to the respective antigens. Washing of the microwells removes unspecific serum and plasma components. Horseradish peroxidase (HRP) conjugated antihuman IgG or IgM immunologically detects the bound patient antibodies forming a conjugate/antibody/antigen complex. Washing of the microwells removes unbound conjugate. An enzyme substrate in the presence of bound conjugate hydrolyzes to form a blue color. The addition of an acid stops the reaction forming a yellow endproduct. The intensity of this yellow color is measured photometrically at 450nm.
Properties
E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays,E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays,Immunoglobulin M, or IgM for short, is a basic antibody that is produced by B cells. IgM is by far the physically largest antibody in the human circulatory system. It is the first antibody to appear in response to initial exposure to an antigen
Test
ELISA Enzyme-linked immunosorbent assays Code 90320007 SNOMED
About
Immunoglobulin gamma, IgG, mouse monoclonal H&L chain clones or rabbit, goat polyclonal antibodies have 4 parts. There are 2 heavy chains, 2 light chains. The IgG antibody has 2 antigen binding sites. They represent 70% or more of serum antibodies. This antibody can be antigen purified or protein A or G purified. For storage sodium azide is added or you can call us to request azide free antibody preparations. These will need colder storage temperatures.
Additional isotype
IgG,IgM