Form & Buffer
This Kit contains: Lyophilized recombinant mouse TNF alpha standard: 10ng/tube ×2, One 96-well plate precoated with anti- mouse TNF alpha antibody, Sample diluent buffer: 30 ml, Biotinylated anti- mouse TNF alpha antibody : 130 ul, dilution 1:100, Antibody diluent buffer: 12ml, Avidin-Biotin-Peroxidase Complex (ABC) : 130 ul, dilution 1:100, ABC diluent buffer: 12ml, TMB color developing agent: 10ml, TMB stop solution: 10ml
Storage
Store at 4 deg C for short term storage (4 months) or -20 deg C for long term storage (8 months)
Assay Information
Sensitivity: 15.6 pg/ml-1000 pg/ml (Body fluids, tissue lysates or cell culture supernates), 7.8 pg/ml-500 pg/ml (Mouse serum)
Description
The Mouse TNF alpha ELISA kit is a α- or alpha protein sometimes glycoprotein present in blood.
Properties
E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays,E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays
Test
ELISA Enzyme-linked immunosorbent assays Code 90320007 SNOMED,Mouse or mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab. Mouse are mature after 40 days for females and 55 days for males. The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year. Transgenic, knock-out, congenic and inbread strains are known for C57BL/6, A/J, BALB/c, SCID while the CD-1 is outbred as strain.
Gene
Tumor necrosis factor (TNFa, tumor necrosis factor alpha, TNFα, cachexin, or cachectin) is a cell signaling protein (cytokine) involved in systemic inflammation and is one of the cytokines that make up the acute phase reaction. It is produced chiefly by activated macrophages, although it can be produced by many other cell types such as CD4+ lymphocytes, NK cells, neutrophils, mast cells, eosinophils, and neurons. TNFb or TNF beta also bin on TNF receptors for Th1 activation.