Storage
Store at 2-8 deg C
Shipping conditions
Blue Ice
Usage Recommendations
Optimal conditions to be determined by end user
Assay Information
An antimouse TNFalpha coating antibody is adsorbed onto microwells. Mouse TNFalpha present in the sample or standard binds to antibodies adsorbed to the microwells. Following incubation unbound biological components are removed during a wash step and a biotinconjugated antimouse TNFalpha antibody is added and binds to mouse TNFalpha captured by the first antibody. Following incubation unbound biotin conjugated antimouse TNFalpha antibody is removed during a wash step. StreptavidinHRP is added and binds to the biotin conjugated antimouse TNFalpha antibody. Following incubation unbound StreptavidinHRP is removed during a wash step, and substrate solution reactive with HRP is added to the wells. A coloured product is formed in proportion to the amount of mouse TNFalpha present in the sample or standard. The reaction is terminated by addition of acid and absorbance is measured at 450 nm. A standard curve is prepared from 7 mouse TNFalpha standard dilutions and mouse TNFalpha sample concentration determined.
Description
The Mouse TNF alpha ELISA kit is a α- or alpha protein sometimes glycoprotein present in blood.
Properties
E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays,E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays
Test
ELISA Enzyme-linked immunosorbent assays Code 90320007 SNOMED,Mouse or mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab. Mouse are mature after 40 days for females and 55 days for males. The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year. Transgenic, knock-out, congenic and inbread strains are known for C57BL/6, A/J, BALB/c, SCID while the CD-1 is outbred as strain.
Gene
Tumor necrosis factor (TNFa, tumor necrosis factor alpha, TNFα, cachexin, or cachectin) is a cell signaling protein (cytokine) involved in systemic inflammation and is one of the cytokines that make up the acute phase reaction. It is produced chiefly by activated macrophages, although it can be produced by many other cell types such as CD4+ lymphocytes, NK cells, neutrophils, mast cells, eosinophils, and neurons. TNFb or TNF beta also bin on TNF receptors for Th1 activation.