Area of research
Hormones & Steroids
Form & Buffer
This Kit contains: Lyophilized recombinant mouse Leptin standard: 10ng/tube ×2, One 96-well plate precoated with anti- mouse Leptin antibody, Sample diluent buffer: 30 ml, Biotinylated anti- mouse Leptin antibody : 130 ul, dilution 1:100, Antibody diluent buffer: 12ml, Avidin-Biotin-Peroxidase Complex (ABC) : 130 ul, dilution 1:100, ABC diluent buffer: 12ml, TMB color developing agent: 10ml, TMB stop solution: 10ml
Storage
Store at 4 deg C for short term storage (4 months) or -20 deg C for long term storage (8 months)
Assay Information
Sensitivity: 62.5 pg/ml-4000 pg/ml
Properties
E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays,E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays
Test
ELISA Enzyme-linked immunosorbent assays Code 90320007 SNOMED,Mouse or mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab. Mouse are mature after 40 days for females and 55 days for males. The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year. Transgenic, knock-out, congenic and inbread strains are known for C57BL/6, A/J, BALB/c, SCID while the CD-1 is outbred as strain.
Gene
Human or mouse Leptin (from Greek λεπτός leptos, "thin") the "satiety hormone", is a hormone made by adipose cells that helps to regulate energy balance by inhibiting hunger. Leptin is opposed by the actions of the hormone ghrelin, the "hunger hormone". Both hormones act on receptors in the arcuate nucleus of the hypothalamus to regulate appetite to achieve energy homeostasis. ELISA kits and peptides and antibodies are available.