All suppliers fitzgerald Mouse IL10 ELISA kit

Information

Catalog number
55R-IB49694
Name
Mouse IL10 ELISA kit
Supplier
Size
96 wells
Price
1130.00

Details

Category
Research kit
Area of research
Cytokines & Growth Factors
Storage
Store at 2-8 deg C
Shipping conditions
Blue Ice
Tested for
ELISA
Usage Recommendations
Optimal conditions to be determined by end user
Assay Information
An antimouse IL10 coating antibody is adsorbed onto microwells. Mouse IL10 present in the sample or standard binds to antibodies adsorbed to the microwells. Following incubation unbound biological components are removed during a wash step and biotinconjugated antimouse IL10 antibody is added and binds to mouse IL10 captured by the first antibody. Following incubation unbound biotin conjugated Following incubation unbound biotin conjugated antimouse IL10 antibody is removed during a wash step. StreptavidinHRP is added and binds to the biotin conjugated antimouse IL10 antibody. antimouse IL10 antibody is removed during a wash step. StreptavidinHRP is added and binds to the biotin conjugated antimouse IL10 antibody. Following incubation unbound StreptavidinHRP is removed during a wash step, and substrate solution reactive with HRP is added to the wells. A coloured product is formed in proportion to the amount of mouse IL10 present in the sample or standard. The reaction is terminated by addition of acid and absorbance is measured at 450 nm. A standard curve is prepared from 7 mouse IL10 standard dilutions and mouse IL10 sample concentration determined.
Properties
E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays,E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays
Test
ELISA Enzyme-linked immunosorbent assays Code 90320007 SNOMED,Mouse or mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab. Mouse are mature after 40 days for females and 55 days for males. The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year. Transgenic, knock-out, congenic and inbread strains are known for C57BL/6, A/J, BALB/c, SCID while the CD-1 is outbred as strain.
Gene
Interleukin 10 (IL-10), also known as human cytokine synthesis inhibitory factor (CSIF), is an anti-inflammatory cytokine. In humans, interleukin 10 is encoded by the IL10 gene. Recombinant. coli interleukin-1o for cell culture or antibody production.
Latin name
Mus musculus