Area of research
Immunology
Storage
Store at 2-8 deg C
Shipping conditions
Blue Ice
Usage Recommendations
Optimal conditions to be determined by end user
Assay Information
The IBLAmerica IgM Isotyping Enzyme Immunometric Assay (EIA) kit is a complete kit for the determintation of mouse IgM in Tissue Culture Media, serum and ascites fluids. Please read the complete kit insert before performing this assay. The kit uses a polyclonal antibody to mouse IgM immobilized on a microtiter plate to bind the mouse IgM in the standards or sample. A mouse IgM Standard is provided in the kit. After a simultaneous incubation with a polyclonal antibody to mouse IgM conjugated to Horseradish peroxidase, which binds to the mouse IgM captured on the plate, the excess reagents are washed out and substrate is added. After a short incubation, the enzyme reaction is stopped and the color generated is read at 450 nm. The measured optical density is directly proportional to the concentration ofmouse IgMin either standards or samples.
Properties
E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays,E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays,Immunoglobulin M, or IgM for short, is a basic antibody that is produced by B cells. IgM is by far the physically largest antibody in the human circulatory system. It is the first antibody to appear in response to initial exposure to an antigen
Test
ELISA Enzyme-linked immunosorbent assays Code 90320007 SNOMED,Mouse or mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab. Mouse are mature after 40 days for females and 55 days for males. The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year. Transgenic, knock-out, congenic and inbread strains are known for C57BL/6, A/J, BALB/c, SCID while the CD-1 is outbred as strain.