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This mouse IgG1 Isotyping Enzyme Immunometric Assay (EIA) kit is a complete kit for the determination of mouse IgG1 in Tissue Culture Media and serum. Please read the complete kit insert before performing this assay. The kit uses a polyclonal antibody to mouse IgG immobilized on a microtiter plate to bind the mouse IgG in the standards or sample. A mouse IgG1 Standard is provided in thekit. After a simultaneous incubation with a polyclonal antibody to mouse IgG1 conjugated to Horseradish peroxidase, which binds to the mouse IgG1 captured on the plate, the excess reagents are washed out and substrate is added. After a short incubation, the enzyme reaction is stopped and the color generated is read at 450 nm. Themeasured optical density is directly proportional to the concentration ofmouse IgG in either standards or samples.
E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays,E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays
ELISA Enzyme-linked immunosorbent assays Code 90320007 SNOMED,Mouse or mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab. Mouse are mature after 40 days for females and 55 days for males. The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year. Transgenic, knock-out, congenic and inbread strains are known for C57BL/6, A/J, BALB/c, SCID while the CD-1 is outbred as strain.