Area of research
Store at 2-8 deg C
Optimal conditions to be determined by end user
Mouse sera/plasma for testing are diluted to 1:20 (suggested) and allowed to react with pneumococcal C polysaccharide coated on specially treated microwells. After appropriate incubation, the wells are washed to remove unreacted serum proteins, and an enzymelabeled rabbit antiCRP (conjugate) is then added to react with and tag the antigenantibody complexes. Following another incubation period, the wells are again washed to remove unreacted conjugate. A urea peroxide substrate with TMB as chromogen is added to start color development. Development of a blue color indicates a positive reaction while negative reactions appear colorless or with a trace of blue. The reaction is interrupted with a stop solution that turns the blue positive reactions to yellow. Negative reactions remain colorless or with a hint of yellow. Color intensity (absorbance) is read at a wavelength of 450 nm on a spectrophotometer or ELISA reader. Semiquantification of absorbance can be accomplished by the use of a standard curve generated by measuring twofold dilutions of the standard provided.
C reactive proteins are detected by anti-CRP antibodies supplied by GENTAUR.
E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays,E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays
ELISA Enzyme-linked immunosorbent assays Code 90320007 SNOMED,Mouse or mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab. Mouse are mature after 40 days for females and 55 days for males. The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year. Transgenic, knock-out, congenic and inbread strains are known for C57BL/6, A/J, BALB/c, SCID while the CD-1 is outbred as strain.