Storage
Store at 2-8 deg C.
Shipping conditions
Blue Ice
Usage Recommendations
Optimal conditions to be determined by end-user
Assay Information
An anti90K/Mac2 BP monoclonal coating antibody is adsorbed onto microwells. 90K/Mac2 BP present in the sample or standard binds to the antibody adsorbed to the microwells. Following incubation a HRPconjugated monoclonal anti90K/Mac2 BP antibody is added and binds to 90K/Mac2 BP captured by the first antibody. Unbound enzyme conjugated anti90K/Mac2 BP is removed during a wash step and substrate solution reactive with HRP is added to the wells. A coloured product is formed in proportion to the amount of soluble 90K/Mac2 BP present in the sample. The reaction is terminated by addition of acid and absorbance is measured at 450nm. A standard curve is prepared from five 90K/Mac2 BP standards and 90K/Mac2 BP sample concentration determined.
Properties
E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays,E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays
Test
ELISA Enzyme-linked immunosorbent assays Code 90320007 SNOMED