All suppliers fitzgerald Influenza A IgG ELISA Kit

Information

Catalog number
55R-RE56511
Name
Influenza A IgG ELISA Kit
Supplier
Size
96 wells
Price
362.00

Details

Category
Research kit
Area of research
Infectious Disease
Storage
Store at 4 deg C.
Shipping conditions
Blue Ice
Tested for
ELISA
Usage Recommendations
Optimal conditions to be determined by end user
Assay Information
The Influenza A IgG antibody test kit is based on the principle of the enzyme immunoassay (EIA). Influenza A antigen is bound on the surface of the microtiter strips. Diluted patient serum or ready-touse standards are pipetted into the wells of the microtiter plate. A binding between the IgG antibodies of the serum and the immobilized Influenza A antigen takes place. After a one hour incubation at room temperature, the plate is rinsed with diluted wash solution, in order to remove unbound material. Then ready-to-use antihuman- IgG peroxidase conjugate is added and incubated for 30 minutes. After a further washing step, the substrate (TMB) solution is pipetted and incubated for 20 minutes, inducing the development of a blue dye in the wells. The color development is terminated by the addition of a stop solution, which changes the color from blue to yellow. The resulting dye is measured spectrophotometrically at the wavelength of 450 nm. The concentration of the IgG antibodies is directly proportional to the intensity of the color.
Virus
influenza
Properties
E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays,E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays
Test
ELISA Enzyme-linked immunosorbent assays Code 90320007 SNOMED
About
Immunoglobulin gamma, IgG, mouse monoclonal H&L chain clones or rabbit, goat polyclonal antibodies have 4 parts. There are 2 heavy chains, 2 light chains. The IgG antibody has 2 antigen binding sites. They represent 70% or more of serum antibodies. This antibody can be antigen purified or protein A or G purified. For storage sodium azide is added or you can call us to request azide free antibody preparations. These will need colder storage temperatures.
Additional isotype
IgG
Description
Influenza A and B H1N1 H3N2 Hemagglutinin-nucleoprotein recombinant proteins, peptides and antibodies detect a virus commonly known as "the flu". Influenza is an infectious disease caused by an influenza virus. Symptoms can be mild to severe. The most common symptoms include a high fever, runny nose, sore throat, muscle pains, headache, coughing, and feeling tired. These symptoms typically begin two days after exposure to the virus and most last less than a week. The cough, however, may last for more than two weeks. In children, there may be nausea and vomiting, but these are not common in adults.