Form & Buffer
This Kit contains: Lyophilized recombinant human TNF alpha standard: 10ng/tube ×2, One 96-well plate precoated with anti- human TNF alpha antibody, Sample diluent buffer: 30 ml, Biotinylated anti- human TNF alpha antibody : 130 ul, dilution 1:100, Antibody diluent buffer: 12ml, Avidin-Biotin-Peroxidase Complex (ABC) : 130 ul, dilution 1:100, ABC diluent buffer: 12ml, TMB color developing agent: 10ml, TMB stop solution: 10ml
Storage
Store at 4 deg C for short term storage (4 months) or -20 deg C for long term storage (8 months)
Assay Information
Sensitivity: 15.6 pg/ml-1000 pg/ml (Body fluids, tissue lysates or cell culture supernates), 7.8 pg/ml-500 pg/ml (Human serum)
Description
The TNF alpha ELISA kit is a α- or alpha protein sometimes glycoprotein present in blood.
Properties
E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays,E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays,Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
Test
ELISA Enzyme-linked immunosorbent assays Code 90320007 SNOMED
Gene
Tumor necrosis factor (TNFa, tumor necrosis factor alpha, TNFα, cachexin, or cachectin) is a cell signaling protein (cytokine) involved in systemic inflammation and is one of the cytokines that make up the acute phase reaction. It is produced chiefly by activated macrophages, although it can be produced by many other cell types such as CD4+ lymphocytes, NK cells, neutrophils, mast cells, eosinophils, and neurons. TNFb or TNF beta also bin on TNF receptors for Th1 activation.