Storage
Store at 2-8 deg C
Shipping conditions
Blue Ice
Usage Recommendations
Optimal conditions to be determined by end user
Assay Information
This human Total Survivin Enzyme Immunometric Assay (EIA) kit is a complete kit for the determination of Survivin in serum, plasma, urine, and cell lysates. Please read the complete kit insert before performing this assay. The kit uses a monoclonal antibody to Survivin immobilized on a microtiter plate to bind the Survivin in the standards or samples. A recombinant Survivin Standard is provided in the kit. After a short incubation the excess sample or standard is washed out and a rabbit polyclonal antibody to Survivin is added. This antibody binds to the Survivin captured on the plate. After a short incubation the excess antibody is washed out and goat antirabbit IgG conjugated to Horseradish peroxidase is added, which binds to the polyclonal Survivin antibody. Excess conjugate is washed out and substrate is added. After a short incubation, the enzyme reaction is stopped and the color generated is read at 450 nm. The measured optical density is directly proportional to the concentration of Survivin in either standards or samples.
Properties
E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays,E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays,Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
Test
ELISA Enzyme-linked immunosorbent assays Code 90320007 SNOMED