An antihuman sCD40L coating antibody is adsorbed onto microwells. Human sCD40L present in the sample or standard binds to antibodies adsorbed to the microwells. A HRPconjugated antihuman sCD40L antibody is added and binds to human sCD40L captured by the first antibody. Following incubation unbound HRP conjugated antihuman sCD40L is removed during a wash step, and substrate solution reactive with HRP is added to the wells. A coloured product is formed in proportion to the amount of human sCD40L present in the sample or standard. The reaction is terminated by addition of acid and absorbance is measured at 450 nm. A standard curve is prepared from 7 human sCD40L standard dilutions and human sCD40L concentration determined.
E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays,E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays,Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.