Area of research
Cytokines & Growth Factors
Storage
Store at 2-8 deg C
Shipping conditions
Blue Ice
Usage Recommendations
Optimal conditions to be determined by end user
Assay Information
An antihuman LIF coating antibody is adsorbed onto microwells. Human LIF present in the sample or standard binds to antibodies adsorbed to the microwells. Following incubation unbound biological components are removed during a wash step. A biotinconjugated antihuman LIF antibody is added and binds to human LIF captured by the first antibody. Following incubation unbound biotin conjugated antihuman LIF antibody is removed during a wash step. Streptavidin HRP is added and binds to the biotinconjugated antihuman LIF antibody. Following incubation unbound Streptavidin HRP is removed during a wash step, and substrate solution reactive with HRP is added to the wells. A coloured product is formed in proportion to the amount of human LIF present in the sample or standard. The reaction is terminated by addition of acid and absorbance is measured at 450 nm. A standard curve is prepared from 7 human LIF standard dilutions and human LIF sample concentration determined.
Properties
E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays,E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays,Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
Test
ELISA Enzyme-linked immunosorbent assays Code 90320007 SNOMED