Area of research
Cytokines & Growth Factors
Form & Buffer
This Kit contains: Lyophilized recombinant human IL-8 standard: 10ng/tube ×2, One 96-well plate precoated with anti- human IL-8 antibody, Sample diluent buffer: 30 ml, Biotinylated anti- human IL-8 antibody : 130 ul, dilution 1:100, Antibody diluent buffer: 12ml, Avidin-Biotin-Peroxidase Complex (ABC) : 130 ul, dilution 1:100, ABC diluent buffer: 12ml, TMB color developing agent: 10ml, TMB stop solution: 10ml
Storage
Store at 4 deg C for short term storage (4 months) or -20 deg C for long term storage (8 months)
Assay Information
Sensitivity: 15.6 pg/ml-1000 pg/ml (body fluids, tissue lysates or cell culture supernates), 7.8 pg/ml-500 pg/ml (human sera, plasma
Properties
E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays,E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays,Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
Test
ELISA Enzyme-linked immunosorbent assays Code 90320007 SNOMED
Gene
Interleukin 8 (IL8 or chemokine (C-X-C motif) ligand 8, CXCL8) is a chemokine produced by macrophages and other cell types such as epithelial cells, airway smooth muscle cells and endothelial cells. Endothelial cells store IL-8 in their storage vesicles, the Weibel-Palade bodies. In humans, the interleukin-8 proteins encoded by the IL8 gene. IL-8 is initially produced as a precursor peptide of 99 amino acids long which then undergoes cleavage to create several active IL-8 isoforms. In culture, a 72 amino acid peptide is the major form secreted by macrophages. Recombinant, rec. E. coli interleukin-8 for cell culture or antibody production.