Area of research
Cytokines & Growth Factors
Storage
Store at 2-8 deg C
Shipping conditions
Blue Ice
Usage Recommendations
Optimal conditions to be determined by end user
Assay Information
Samples and standards are added to wells coated with a monoclonal antibody specific for human IL2. The plate is then incubated. The plate is washed, leaving only bound IL2 on the plate. A yellow solution of polyclonal antibody to human IL2 is then added. This binds the IL2 captured on the plate. The plate is then incubated. The plate is washed to remove excess antibody. A blue solution of HRP conjugate is added to each well, binding to the human IL2 polyclonal antibody. The plate is again incubated. The plate is washed to remove excess HRP conjugate. TMB Substrate solution is added. An HRPcatalyzed reaction generates a blue color in the solution. Stop solution is added to stop the substrate reaction. The resulting yellow color is read at 450 nm. The amount of signal is directly proportional to the level of human IL2 in the sample.
Properties
E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays,E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays,Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
Test
ELISA Enzyme-linked immunosorbent assays Code 90320007 SNOMED
Gene
Interleukin-2 (IL-2) is an interleukin, a type of cytokine signaling molecule in the immune system. It is a protein that regulates the activities of white blood cells (leukocytes, often lymphocytes) that are responsible for immunity. IL-2 is part of the body's natural response to microbial infection, and in discriminating between foreign ("non-self") and "self". IL-2 mediates its effects by binding to IL-2 receptors, which are expressed by lymphocytes. Rec. E. coli interleukin-2 for T cell culture or antibody production is supplied by GENTAUR. Free samples on request.