Area of research
Cytokines & Growth Factors
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An antiIL12 polyclonal coating antibody is adsorbed onto microwells. IL12 present in the sample or standard binds to antibodies adsorbed to the microwells; a biotinconjugated polyclonal antiIL12 antibody is added and binds to IL12 captured by the first antibody. Following incubation unbound biotin conjugated antiIL12 is removed during a wash step. StreptavidinHRP is added and binds to the biotin conjugated antiIL12. Following incubation unbound StreptavidinHRP is removed during a wash step, and substrate solution reactive with HRP is added to the wells. A coloured product is formed in proportion to the amount of IL12 present in the sample. The reaction is terminated by addition of acid and absorbance is measured at 450nm. A standard curve is prepared from seven IL12 standard dilutions and IL12 sample concentration determined.
E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays,E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays,Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
ELISA Enzyme-linked immunosorbent assays Code 90320007 SNOMED