Area of research
Cytokines & Growth Factors
Storage
Store at 2-8 deg C
Shipping conditions
Blue Ice
Usage Recommendations
Optimal conditions to be determined by end user
Assay Information
An antihuman IL10 coating antibody is adsorbed onto microwells. Human IL10 present in the sample or standard binds to antibodies adsorbed to the microwells. A biotinconjugated antihuman IL10 antibody is added and binds to human IL10 captured by the first antibody. Following incubation unbound biotin conjugated antihuman IL10 antibody is removed during a wash step. StreptavidinHRP is added and binds to the biotin conjugated antihuman IL10 antibody. Following incubation unbound StreptavidinHRP is removed during a wash step, and substrate solution reactive with HRP is added to the wells.
Properties
E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays,E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays,Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
Test
ELISA Enzyme-linked immunosorbent assays Code 90320007 SNOMED
Gene
Interleukin 10 (IL-10), also known as human cytokine synthesis inhibitory factor (CSIF), is an anti-inflammatory cytokine. In humans, interleukin 10 is encoded by the IL10 gene. Recombinant. coli interleukin-1o for cell culture or antibody production.