All suppliers fitzgerald Human IL1 beta ELISA kit

Information

Catalog number
55R-IB49624
Name
Human IL1 beta ELISA kit
Supplier
Size
96 wells
Price
1246.00

Details

Category
Research kit
Area of research
Cytokines & Growth Factors
Storage
Store at 2-8 deg C
Shipping conditions
Blue Ice
Tested for
ELISA
Usage Recommendations
Optimal conditions to be determined by end user
Assay Information
An antihuman IL1 beta coating antibody is adsorbed onto microwells. Human IL1 beta present in the sample or standard binds to antibodies adsorbed to the microwells. A biotinconjugated antihuman IL1 beta antibody is added and binds to human IL1 beta captured by the first antibody. Following incubation unbound biotin conjugated antihuman IL1 beta antibody is removed during a wash step. StreptavidinHRP is added and binds to the biotinconjugated antihuman IL1 beta antibody. Following incubation unbound StreptavidinHRP is removed during a wash step, and substrate solution reactive with HRP is added to the wells. A coloured product is formed in proportion to the amount of human IL1 beta present in the sample or standard. The reaction is terminated by addition of acid and absorbance is measured at 450 nm. A standard curve is prepared from 7 human IL1 beta standard dilutions and human IL1 beta sample concentration determined.
Properties
E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays,E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays,Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
Test
ELISA Enzyme-linked immunosorbent assays Code 90320007 SNOMED