Area of research
Protein Modification & Stress Response
Storage
Store at 2-8 deg C
Shipping conditions
Blue Ice
Usage Recommendations
Optimal conditions to be determined by end user
Assay Information
HSP60 (human), EIA is a sandwich immunoassay. A mouse monoclonal antibody specific for Hsp60 is precoated on the wells of the provided AntiHsp60 Immunoassay Plate. Hsp60 is captured by the immobilized antibody and is detected with an Hsp60 specific, goat polyclonal antibody. The goat polyclonal antibody is subsequently bound by a horseradish peroxidase conjugated antigoat IgG secondary antibody. The assay is developed with tetramethylbenzidine substrate and a blue color develops in proportion to the amount of captured Hsp60. The color development is stopped with acid stop solution which converts the endpoint color to yellow. The intensity of the color is measured in a microplate reader at 450nm. Hsp60 concentrations from the sample are determined by interpolating absorbance readings from a standard curve generated with the calibrated Hsp60 protein standard provided.
Properties
E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays,E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays,Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
Test
ELISA Enzyme-linked immunosorbent assays Code 90320007 SNOMED