This human Gastrin I kit is a competitive immunoassay for the determination of Gastrin in samples. The kit uses a polyclonal antibody to Gastrin I to bind, in a competitive manner, the Gastrin in the sample or an alkaline phosphatase molecule which has Gastrin I covalently attached to it. After a simultaneous incubation at roomtemperature the excess reagents are washed away and substrate is added. After a short incubation time the enzyme reaction is stopped and the yellow color generated read on a microplate reader at 405nm. The intensity of the bound yellow color is inversely proportional to the concentration of Gastrin I in either standards or samples. Themeasured optical density is used to calculate the concentration of Gastrin I.
E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays,E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays,Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.