Research kit

Proteases, Inhibitors, & Enzymes

Store at 2-8 deg C

Blue Ice

ELISA

Optimal conditions to be determined by end-user

An antihuman Caspase9 coating antibody is adsorbed onto microwells. Human Caspase9 present in the sample or  standard binds to antibodies adsorbed to the microwells. The polyclonal detection antibody (rabbit) binds to human Caspase9 captured by the first antibody. Following incubation unbound detection antibody is removed during a wash step. AntirabbitIgGHRP is added and binds to the detection Antibody. Following incubation unbound antirabbit  IgGHRP is removed during a wash step, and substrate solution reactive with HRP is added to the wells. A coloured product is formed in proportion to the amount of human Caspase9 present in the sample or standard. The reaction is  terminated by addition of acid and absorbance is measured at 450 nm. A standard curve is prepared from 7 human  Caspase9 standard dilutions and human Caspase9 concentration determined.

Human and some mouse caspases are active in apoptosis and cell death and even in necrosis and inflammation. CASP Gene and orthologous enzymes have been identifies successfully in the signal transduction cascade and pathways.

Human and some mouse caspases are active in apoptosis and cell death and even in necrosis and inflammation. CASP Gene and orthologous enzymes have been identifies successfully in the signal transduction cascade and pathways.

E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays,E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays,Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.

ELISA Enzyme-linked immunosorbent assays Code 90320007 SNOMED