All suppliers fitzgerald Huma sVE Cadherin ELISA kit

Information

Catalog number
55R-IB49647
Name
Huma sVE Cadherin ELISA kit
Supplier
Size
96 wells
Price
1246.00
Buy

Details

Category
Research kit
Area of research
Cell Biology
Storage
Store at 2-8 deg C
Shipping conditions
Blue Ice
Tested for
ELISA
Usage Recommendations
Optimal conditions to be determined by end user
Assay Information
An antihuman VEcadherin coating antibody is adsorbed onto microwells. Human VEcadherin present in the sample or  standard binds to antibodies adsorbed to the microwells. A biotinconjugated antihuman VEcadherin antibody is added and binds to human VEcadherin captured by the first antibody. Following incubation unbound biotin conjugated  antihuman VEcadherin antibody is removed during a wash step. StreptavidinHRP is added and binds to the biotin conjugated antihuman VEcadherin antibody. Following incubation unbound StreptavidinHRP is removed during a wash step, and substrate solution reactive with HRP is added to the wells. A coloured product is formed in proportion to the amount of human VEcadherin present in the sample or standard. The reaction is terminated by addition of acid and absorbance is measured at 450 nm. A standard curve is prepared from 7 human VEcadherin standard dilutions and human VEcadherin sample concentration determined.
Properties
E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays,E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays
Test
ELISA Enzyme-linked immunosorbent assays Code 90320007 SNOMED