Area of research
Immunology
Storage
Store at 2-8 deg C.
Shipping conditions
Blue Ice
Usage Recommendations
Optimal conditions to be determined by end-user
Assay Information
Heparinized whole blood samples are incubated with different concentrations of the suspected allergen. Release of histamine will occur upon stimulation of basophilic granulocytes depending on their sensibility to the allergen. The released histamine in the supernatant is subsequently determined using a specific plasma immunoassay for the detection of histamine purchased in connection with this kit. This histamine value is related to the 100% control (= Total Histamine) and the blank value (= Spontaneous Release). The determination of the in vitro release of histamine represents a sensitive and specific method as well as a suitable addition to routine diagnostic procedures including conventional skin testing and radioallergosorbent tests (RAST) for the determination of specific IgE anbodies in serum of atopic patients. In addition, this test also detects the “releasability“ of the cells.
Properties
E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays,E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays
Test
ELISA Enzyme-linked immunosorbent assays Code 90320007 SNOMED