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Store at 2-8 deg C.
Optimal conditions to be determined by end-user
The assay kit provides materials for the quantitative determination of derivatized histamine in food extracts. The derivatization is part of the preparation of the samples. By using the acylation reagent, histamine is derivatized into Nacylhistamine. The competitive Histamine EIA kit uses the microtiter plate format. Histamine is bound to the solid phase of the microtiter plate. Acylated histamine and solid phase bound histamine compete for a fixed number of antiserum binding sites. When the system is in equilibrium, free antigen and free antigenantiserum complexes are removed by washing. The antibody bound to the solid phase histamine is detected by antigoat/peroxidase. The substrate TMB/peroxidase reaction is monitored at 450 nm. The amount of antibody bound to the solid phase histamine is inversely proportional to the histamine concentration of the sample.
When using milk samples, precipitator 85R-IB89141 is recommended for pre-treatment.
E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays,E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays
ELISA Enzyme-linked immunosorbent assays Code 90320007 SNOMED