Area of research
Infectious Disease
Storage
Store at 2-8 deg C
Shipping conditions
Blue Ice
Usage Recommendations
Optimal conditions to be determined by end user
Assay Information
This Helicobacter pylori IgA antibody test kit is based on the principle of the enzyme immunoassay (EIA). Helicobacter antigen is bound on the surface of the microtiter strips. Diluted patient serum or readytouse standards are pipetted into the wells of the microtiter plate. A binding between the IgA antibodies of the serum and the immobilized Helicobacter antigen takes place. After a one hour incubation at room temperature, the plate is rinsed with diluted wash solution, in order to remove unbound material. Then readytouse antihumanIgA peroxidase conjugate is added and incubated for 30 minutes. After a further washing step, the substrate (TMB) solution is pipetted and incubated for 20 minutes, inducing the development of a blue dye in the wells. The color development is terminated by the addition of a stop solution, which changes the color from blue to yellow. The resulting dye is measured spectrophotometrically at the wavelength of 450 nm. The concentration of the IgA antibodies is directly proportional to the intensity of the color.
Properties
E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays,E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays,Immunoglobulin A (IgA, also referred to as sIgA) is an antibody that plays a critical role in immune function in the mucous membranes. More IgA is produced in mucosal linings than all other types of antibody combined. Examples are EMA, ACA ELISA test kits and for diagnostic detection the IgA of Anti-Beta-2 Glycoprotein 1; β2-Glycoprotein 1 Antibodies; Beta 2GP1 Ab, Beta-2 Glycoprotein 1 Antibodies IgG, IgM, and IgAs.
Test
ELISA Enzyme-linked immunosorbent assays Code 90320007 SNOMED
Additional disease
Helicobacter pillory antibodies and ELISA kits as diagnostic to detect Gram-negative helicobacter bacteria genus name Helicobacter.