Research kit

Proteases, Inhibitors, & Enzymes

Store at 2-8 deg C

Blue Ice

ELISA

Optimal conditions to be determined by end user

This Total Glycogen Synthase Kinase (GSK)3B Enzyme Immunometric Assay (EIA) kit is a complete kit for the determination of pan or total GSK3B in cell lysates. Please read the complete kit insert before performing this assay. The kit uses a monoclonal antibody to GSK3B immobilized on a microtiter plate to bind the GSK3B in the standards or sample. A recombinant GSK3B Standard is provided in the kit. After a short incubation, the excess sample or standard is washed out and a rabbit polyclonal antibody to GSK3B is added. This antibody binds to GSK3B captured on the plate. The polyclonal antibody detects a nonphosphorylated region of GSK3B and therefore detects GSK3B regardless of phosphorylation status. After a short incubation, the excess antibody is washed out and goat antirabbit IgG conjugated to horseradish peroxidase is added,which binds to the polyclonal GSK3B antibody. Excess conjugate is washed out and substrate is added. After a short incubation, the enzyme reaction is stopped and the color generated is read at 450 nm. The measured optical density is directly proportional to the concentration of GSK3B in either standards or samples.

E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays,E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays

ELISA Enzyme-linked immunosorbent assays Code 90320007 SNOMED