Area of research
Hormones & Steroids
Storage
Store at 2-8 deg C
Shipping conditions
Blue Ice
Usage Recommendations
Optimal conditions to be determined by end user
Assay Information
Total Estriol (antigen) in the sample competes with horseradishperoxidase Estriol (enzymelabelledantigen) for binding onto the limited number of anti Estriol (antibody) sites on the microplates (solid phase). After incubation, the bound/total separation is performed by a simple solidphase washing. The enzyme substrate (H2O2) and the TMBSustrate (TMB) are added. After an appropriate time has elapsed for maximum colour development, the enzyme reaction is stopped and the absorbances are determined. Total Estriol concentration in the sample is calculated based on a series of standard. The colour intensity is inversely proportional to the Total Estriol concentration in the sample.
Properties
E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays,E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays
Test
ELISA Enzyme-linked immunosorbent assays Code 90320007 SNOMED