Area of research
Hormones & Steroids
Storage
Store at 4 deg C.
Shipping conditions
Blue Ice
Usage Recommendations
Optimal conditions to be determined by end user
Assay Information
The free cortisol in saliva ELISA kit is a solid phase ELISA based on the principle of competitive binding. The microtiter wells are coated with a mouse monoclonal antibody directed towards an antigenic site on the Cortisol molecule. Endogenous Cortisol of a patient sample competes with a Cortisol-horseradish peroxidase conjugate for binding to the coated antibody. After incubation the unbound conjugate is washed off. The amount of bound peroxidase conjugate is inversely proportional to the concentration of Cortisol in the sample. After addition of the substrate solution, the intensity of colour developed is reverse proportional to the concentration of Cortisol in the patient sample.
Properties
E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays,E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays
Test
ELISA Enzyme-linked immunosorbent assays Code 90320007 SNOMED