All suppliers fitzgerald CaspSCREEN Flow Cytometric Apoptosis Detection Kit

Information

Catalog number
55R-1301
Name
CaspSCREEN Flow Cytometric Apoptosis Detection Kit
Supplier
Size
100 assays
Price
688.00

Details

Category
Research kit
Area of research
Cell Cycle & Cell Death
Form & Buffer
Assay Kit containing the folllowing: D₂R Reagent, DTT (1 M), D₂R Incubation Buffer
Storage
Store at -20 deg C
Shipping conditions
Blue Ice
Tested for
FC; Functional Assay
Additional Information
CaspSCREEN is a registered trademark
Description
This 1 is suited for programmed cell-death studies.The detections of the targets with this kit is a type of test that can be performed on any target containing biological samples after clean up of interfering agents. The assay must be performed following the protocol.
Test
Flow cytometry uses monoclonal antibodies of specific affinity clones for cell counting, cell sorting and biomarker detection by suspending cells in a stream of fluid for Forward Scatter, FSC and side scatter, SSC analysis. Human PBMCs can be loaded with CFSE tracking dye after non adherent cell harvesting. Subsequently labeled with anti-CD antibodies, and analyzed by multiparameter flow cytometry. Two-parameter profiles of CD vs. CFSE; and another CD vs. FSC-W. We suggest to use FSC-H vs. FSC-A. FSC-A, FSC-H, FSC-W = area, height, and width of the forward 488 nm light scatter from the flow signal.