Area of research
Store at 2-8 deg C
Optimal conditions to be determined by end user
Canine sera for testing are diluted to 1:500 and allowed to react with pneumococcal Cpolysaccharide coated on specially treated microwells. After appropriate incubation, the wells are washed to remove unreacted serum proteins, and an enzymelabeled goat antidog CRP (conjugate) is then added to react with and tag the antigenantibody complexes. Following another incubation period, the wells are again washed to remove unreacted conjugate. A urea peroxide substrate with TMB as chromogen is added to start color development. Development of a blue color indicates a positive reaction while negative reactions appear colorless or with a trace of blue. The reaction is interrupted with a stop solution that turns the blue positive reactions to yellow. Negative reactions remain colorless or with a hint of yellow. Color intensity (absorbance) is read at a wavelength of 450nm on a spectrophotometer or ELISA reader. Assigning a value for the absorbance can be accomplished by the use of a standard curve generated by measuring fourfold dilutions of the standard provided.
C reactive proteins are detected by anti-CRP antibodies supplied by GENTAUR.
E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays,E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays
ELISA Enzyme-linked immunosorbent assays Code 90320007 SNOMED