Information

Catalog number
55R-IB49674
Name
APRIL ELISA kit
Supplier
Size
96 wells
Price
1246.00

Details

Category
Research kit
Area of research
Cytokines & Growth Factors
Storage
Store at 2-8 deg C
Shipping conditions
Blue Ice
Tested for
ELISA
Usage Recommendations
Optimal conditions to be determined by end-user
Assay Information
An antihuman APRIL coating antibody is adsorbed onto microwells. Human APRIL present in the sample or standard binds to antibodies adsorbed to the microwells. A biotinconjugated antihuman APRIL antibody is added and binds to human APRIL captured by the first antibody. Following incubation unbound biotinconjugated antihuman APRIL antibody is removed during a wash step. StreptavidinHRP is added and binds to the biotinconjugated antihuman APRIL antibody. Following incubation unbound StreptavidinHRP is removed during a wash step, and substrate solution reactive with HRP is added to the wells. A coloured product is formed in proportion to the amount of human APRIL present in the sample or standard. The reaction is terminated by addition of acid and absorbance is measured at 450 nm. A standard curve is prepared from 7 human APRIL standard dilutions and human APRIL sample concentration determined.
Properties
E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays,E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays
Test
ELISA Enzyme-linked immunosorbent assays Code 90320007 SNOMED