Area of research
Store at 2-8 deg C
Optimal conditions to be determined by end-user
An antihuman Annexin V coating antibody is adsorbed onto microwells. Human Annexin V present in the sample or standard binds to antibodies adsorbed to the microwells. A biotinconjugated antihuman Annexin V antibody is added and binds to human Annexin V captured by the first antibody. Following incubation unbound biotinconjugated antihuman Annexin V antibody is removed during a wash step. StreptavidinHRP is added and binds to the biotinconjugated antihuman Annexin V antibody. Following incubation unbound StreptavidinHRP is removed during a wash step, and substrate solution reactive with HRP is added to the wells. A coloured product is formed in proportion to the amount of human Annexin V present in the sample or standard. The reaction is terminated by addition of acid and absorbance is measured at 450 nm. A standard curve is prepared from 7 human Annexin V standard dilutions and human Annexin V sample concentration determined.
This Annexin V calcium dependent phospholipid binding protein will be downstream of BCL2 pathway and at caspase apoptotic level. fitzgerald has more cell death kits and antibodies.
E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays,E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays
ELISA Enzyme-linked immunosorbent assays Code 90320007 SNOMED