Area of research
Cell Cycle & Cell Death
Form & Buffer
Assay Kit containing the folllowing: Annexin V-EGFP, 1X Binding Buffer, Propidium Iodide (PI)
Shipping conditions
Blue Ice
Tested for
FC; IF; Functional Assay
Description
This Annexin V calcium dependent phospholipid binding protein will be downstream of BCL2 pathway and at caspase apoptotic level. fitzgerald has more cell death kits and antibodies.This 1 is suited for programmed cell-death studies.The detections of the targets with this kit is a type of test that can be performed on any target containing biological samples after clean up of interfering agents. The assay must be performed following the protocol.
Conjugate
The Green Fluorescent Protein (eGFP) of Annexin V Apoptosis Kit can be excited at 488 nanometers with a peak emission at 509 nanometers and is detected in the FL1 detector on the FACSCalibur or FACScan. The LSRII and all of the core's sorter flow cytometers can tell the difference between at the same time/together expressing eGFP and eYFP cells when the proper optical filters and experimental controls exist.