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Highly purified antigens are bound to nitrocellulose membrane strips. Antibodies against these antigens, if present in diluted serum or plasma, bind to the respective antigen. Washing of the membrane strips removes unspecific serum and plasma components. Alkaline phosphatase conjugated antihuman IgG immunologically detects the bound patient antibodies forming a conjugate/antibody/antigen complex. Washing of the membrane strips removes unbound conjugate. An enzyme substrate in the presence of bound conjugate hydrolyses to form an insoluble blueviolet product. Washing of the membrane strips removes unhydrolysed substrate. The intensity of the colour is directly proportional to the concentration of IgG antibodies present in the original sample.
E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays,E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays
ELISA Enzyme-linked immunosorbent assays Code 90320007 SNOMED