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AMAM2 is an indirect solid phase enzyme immunometric assay (ELISA). It is designed for the quantitative measurement of IgG class autoantibodies directed against mitochondrial M2 subtype antigen. The assay is based on microplates coated with mitochondrial M2 antigen. The microplates can be divided into 12 modules of 8 wells each or can be used completely for 96 determinations. Each well can be separated from the module ("breakaway"). During this procedure the binding of present autoantibodies, as well as the formation of the sandwich complexes and enzymatic colour reaction take place during three different reaction phases. The amount of colour is directly proportional to the concentration of IgG present in the original sample. The optical density for each calibrator may be graphically plotted against the concentration of IgG and unknowns extrapolated from the curve. To read the optical density a microplate reader with a 450 nm filter is required. Bichromatic measurement with a 600650 nm reference is recommended.
E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays,E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays
ELISA Enzyme-linked immunosorbent assays Code 90320007 SNOMED