Store at 2-8 deg C
Optimal conditions to be determined by end user
The AFP is a direct solid phase sandwich ELISA method. The samples and diluted antiAFPHRP conjugate are added to the wells coated with MAb to beta subunit. AFP in the patient’s serum binds to antiAFP MAb on the well and the antiAFP second antibody then binds to AFP. Unbound protein and HRP conjugate are washed off by wash buffer. Upon the addition of the substrate, the intensity of color is proportional to the concentration of AFP in the samples. A standard curve is prepared relating color intensity to the concentration of the AFP.
E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays,E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays
ELISA Enzyme-linked immunosorbent assays Code 90320007 SNOMED