Research kit

Hormones & Steroids

Store at 2-8 deg C.

Blue Ice

ELISA

Optimal conditions to be determined by end-user

First, the plasma proteins are removed by precipitation. After this Metanephrine (Metadrenaline) and Normetanephrine (Normetadrenaline) are acylated. The subsequent competitive ELISA kit uses the microtiter plate format. The antigen is bound to the solid phase of the microtiter plate. The acylated standards, controls and samples and the solid phase bound analytes compete for a fixed number of antiserum binding sites. After the system is in equilibrium, free antigen and free antigenantiserum complexes are removed by washing. The antibody bound to the solid phase is detected by an antirabbit IgGperoxidase conjugate using TMB as a substrate. The reaction is  monitored at 450 nm. Determination of unknown samples is achieved by comparing their absorbance with a reference curve prepared with known standards. The antisera used in this test kit only recognise the biologically relevant Lforms of metanephrines. Commercially available synthetic normetanephrine or metanephrine is always a mixture of the Dand Lforms. The ratio between both forms differs widely from lot to lot. This has important implications if synthetic metanephrines are used to enrich native samples. As only about 50% of the synthetic metanephrines  the Lportion  will be detected by use of this kit, spiked samples will be underestimated. Therefore native samples containing solely the Lform should be used.

E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays,E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays

ELISA Enzyme-linked immunosorbent assays Code 90320007 SNOMED