All suppliers fitzgerald Cardiolipin IgG/IgM1 ELISA kit

Information

Catalog number
55R-ORG515
Name
Cardiolipin IgG/IgM1 ELISA kit
Supplier
Size
96 wells
Price
449.00

Details

Category
Research kit
Area of research
Immunology
Storage
Store at 2-8 deg C
Shipping conditions
Blue Ice
Tested for
ELISA
Usage Recommendations
Optimal conditions to be determined by end-user
Assay Information
Highly purified cardiolipin is bound to microwells saturated with beta2glycoprotein I. Antibodies to these antigens, if present in diluted serum, bind in the microwells. Washing of the microwells removes unbound serum antibodies. Horseradish peroxidase (HRP) conjugated antihuman IgG and IgM immunologically bind to the bound patient antibodies forming a conjugate/antibody/antigen complex. Washing of the microwells removes unbound conjugate. An enzyme substrate in the presence of bound conjugate hydrolyzes to form a blue color. The addition of an acid stops the reaction forming a yellow endproduct. The intensity of this yellow color is measured photometrically at 450 nm. The amount of colour is directly proportional to the concentration of IgG resp. IgM antibodies present in the original sample.
Properties
E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays,E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays
Test
ELISA Enzyme-linked immunosorbent assays Code 90320007 SNOMED
About
Immunoglobulin gamma, IgG, mouse monoclonal H&L chain clones or rabbit, goat polyclonal antibodies have 4 parts. There are 2 heavy chains, 2 light chains. The IgG antibody has 2 antigen binding sites. They represent 70% or more of serum antibodies. This antibody can be antigen purified or protein A or G purified. For storage sodium azide is added or you can call us to request azide free antibody preparations. These will need colder storage temperatures.
Additional isotype
IgG